An Investigation on the Bioavailability of SBR Bioreactor Enzyme Inhibitor Induced with Hydrogen Peroxide

Abstract Background & aim: S-triazine, is one of the most widely used herbicides of estrazines, and             S-triazine, contains of atrazine, amtryn and prom ether. S-triazine is one of the most stable herbicides that pollute water resources.  This material is used for controlling broad-leaved weeds and corn. The purpose of this study was to determine the bioavailability of SBR bioreactor enzyme inhibitor, which was stimulated with hydrogen peroxide.   Methods: in this biodegradation enzyme study which took place in 2017, the toxin-induced enzymatic biodegradation of atrazine in a sequencing batch reactor (SBR) was performed. Synthetic sewage was prepared by dissolving 1 gram of atrazine powder in 1000 ml distilled water. Different stages of the experiment included enrichment of atrazine degrading bacteria and determination of initial concentration of atrazine (10025 mg / l), determination of the effect of hydrogen peroxide concentration (H2O2), determination of reaction time and microbial activity. The pH of the test was stationary at 7. The concentration of atrazine was measured by HPLC.   Results: The validity of this study was 72 days. The number of days required to completely eliminate atrazine was reduced and from 32 days in the first stage, it was reduced to 1 day. The optimal H2O2 / ATZ ratio was 11, with atrazine concentration of 25 mg / L with a concentration of 7.5 mmol per hour at the reaction time (HRT) of 24 hours completely eliminated. In atrazine concentrations, 25 and 100 mg / l were eliminated at 15 and 54 hours, respectively. With increasing atrazine concentration from 25 mg/l to 50 mg / l, the activity of the enzyme dehydrogenase increased from 8.8 to +9.7 TF/g biomass.d. was increased.   Conclusion: Based on the results of this study, atrazine decomposition using peroxidase enzyme in the presence of hydrogen peroxide is an effective and novel biodegradable enzyme for the complete decomposition and removal of this pollutant from contaminated water and sewage.